11/9/2023 0 Comments Licor western blotTrueBlack® WB Blocking Buffer performs as well or better for fluorescent WB compared to LI-COR’s Odyssey® Blocking Buffer (Figure 1), and is priced lower on a per membrane basis.ĭraq5 is a trademark of BioStatus Ltd. Switch from Odyssey® Blocking Buffer and save TrueBlack® blocking buffer is especially advantageous for phosphoprotein detection, significantly improving specificity compared to conventional blocking buffers (Figure 1). The TrueBlack® WB Blocking Buffer Kit blocks background from multiple sources including charged dye conjugates (Figure 2). However, the extra charge carried by antibodies labeled with these dyes can result in non-specific binding to proteins and membranes. Highly charged dyes like Alexa Fluor® or CF® dyes have improved solubility and brightness of conjugates compared to uncharged dyes. Another potential cause of background is the effect of fluorescent dyes themselves on the specificity of labeled antibodies. ![]() Non-specific signal in WB can arise from multiple sources, including antibody cross-reactivity with off-target proteins, non-specific antibody adsorption to the membrane, and membrane autofluorescence. Superior western blocking for next-generation fluorescent dyes Near-IR CF Dye Secondary Antibodies for Multiplex WB Conjugateĭon’t see what you’re looking for? Contact us! We may be able to add a new conjugate to our catalog, or perform a custom labeling for you.īv: bovine, Ck: chicken, GP: guinea pig, Gt: goat, Hs: horse, Hu: human, Ms: mouse, Rb: rabbit, Rt: rat, SHm: Syrian hamster, Shp: sheep, Sw: swineīack to top TrueBlack® WB Blocking Buffer Kit
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